For adjuvant-induced arthritis (AIA) rats, paw edema, mechanical withdrawal threshold, serum levels of TNF- α and IL-10, and histopathological changes of the affected paw were assessed. We measured time of licking the affected paw in formaldehyde-induced inflammatory model, paw volume in egg-white-induced inflammation, and granuloma weight in formalin-soaked filter paper-induced granuloma. Rheumatoid arthritis-associated models, the denatured NNAV (heat treated 30, 90, 270 μg/kg), the native NNAV (untreated with heat 90 μg/kg), and Tripterygium wilfordii polyglycoside (TWP, 15 mg/kg) were administrated orally either prophylactically or therapeutically. To investigate the antinociceptive and anti-inflammatory activities of the denatured Naja Naja atra venom (NNAV) in
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